Strain identification inRhizobium trifoliiusing DNA restriction analysis, plasmid DNA profiles and intrinsic antibiotic resistances

Characterization of Avian Pasteurella multocida Isolates by Protein Profiles and Restriction Enzyme Analysis of Chromosomal DNA

DNA restriction-modification systems mediate plasmid maintenance.

Two plasmid-carried restriction-modification (R-M) systems, EcoRI (from pMB1 of Escherichia coli) and Bsp6I (from pXH13 of Bacillus sp. strain RFL6), enhance plasmid segregational stability in E. coli and Bacillus subtilis, respectively. Inactivation of the endonuclease or the presence of the methylase in trans abolish the stabilizing activity of the R-M systems. We propose that R-M systems med...

characterization of avian pasteurella multocida isolates by protein profiles and restriction enzyme analysis of chromosomal dna

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Molecular Identification Of Probiotics Lactobacillus Strain isolates by : Amplified Ribosomal DNA Restriction Analysis ( ARDRA )

DRA) ,” Science Journal of Microbiology,Volume 2012, Article ID SJMB-175, 8 Pages, 2012. doi:10.7237/sjmb/175 Abstract–In recent years interest in the probiotic lactobacilli has been stimulated by the use of these bacteria in products that are claimed to confer health benefits on the consumer. The objective of this study is to characterize probiotic Lactobacillus sp isolated from fermented mill...

CEA Plasmid as Therapeutic DNA Vaccination against Colorectal Cancer

Background: Human colorectal cancer cells overexpress carcinoembryonic antigen (CEA). CEA is a glycoprotein which has shown to be a promising vaccine target for immunotherapy against colorectal cancer. Objective: To design a DNA vaccine harboring CEA antigen and evaluate its effect on inducing immunity against colorectal cancer cells in tumor bearing mice. <str...